目的：优化哺乳动物细胞表达系统，提高目的基因的表达效率。
Objective: to increase the expression level of foreign gene by the optimization of the expressional regulatory elements in the cassette of interesting gene.
结论构建了人瘦素的哺乳动物细胞表达载体，并成功地在COS 7细胞中获得重组人瘦素的分泌表达。
Conclusion Cloning and expression recombinants of human leptin are obtained and secretive recombinant human leptin is successfully expressed in COS-7 cells.
在哺乳动物细胞里，启动子是一个包含着许多蛋白质结合位点的复杂区域，它调节基因的表达。
In mammalian cells, the promoter is a complex region containing binding sites for many proteins that regulate gene expression.
结果表明，人工转录因子能有效地促进外源基因在哺乳动物细胞中的表达。
These results indicated that artificial transcription factor is potent in the enhancement of exogenous gene expression in mammalian cells.
在细胞生物学中用嘌罗霉素来选择用载体转化能表达嘌呤霉素- N -乙酰基转移酶类的哺乳动物细胞系。
Puromycin is used in cell biology to select mammalian cell lines that have been transformed by vectors that express puromycin-N-acetyl-transferase.
基因在哺乳动物细胞中的表达效率受到细胞状态的影响，研究图案化细胞的基因转染情况对于基因治疗有着重要的意义。
Gene expression in mammalian cells will be influenced by the cell status. Consequently, gene transfection studies on patterned cells are of great meaning for their gene therapeutic applications.
基因在哺乳动物细胞中的表达效率受到细胞状态的影响，研究图案化细胞的基因转染情况对于基因治疗有着重要的意义。
Gene expression in mammalian cells will be influenced by the cell status. Consequently, gene transfection studies on patterned cells are of great meaning for their gene therapeutic applications.
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