定点突变是研究基因功能的一种关键方法。
Site-directed mutagenesis is one of the key methods used to study gene function.
它提供一种简单的方法来实现关键功能和非功能性需求，不需要编程，也不需要使用限定符对服务实现代码进行修改。
It provides an easy way to achieve critical functional and non-functional requirements, without requiring programming or any change to service implementation code by means of qualifiers.
关键是要选择一种方法，构建评估框架，并且在其应用中保持一致。
The key is to pick a method, build an estimation framework, and be consistent with its application.
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