所谓电穿孔(Electroporation)即是在外加短时强电脉冲时,在细胞膜双脂层上形成瞬时微孔,使细胞膜的通透性增强,离子,亲水分子,病毒颗粒,DNA,蛋白质以及染...
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”他负责治疗中使用的电穿孔法技术。 电穿孔法(Electroporation)就是用一个手持设备对皮肤施加电脉冲,在癌细胞膜上开出小孔来。治疗性的小分子就会在小孔重新闭合之前通过小孔滑入癌细胞。
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”他负责治疗中使用的电穿孔法技术。 电穿孔法(Electroporation)就是用一个手持设备对皮肤施加电脉冲,在癌细胞膜上开出小孔来。治疗性的小分子就会在小孔重新闭合之前通过小孔滑入癌细胞。
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...电穿孔转染;流式细胞术;人T淋巴细胞 [gap=844]Key words】 Chimeric immune receptor; Carcinoembryonic antigen; Electroporation; Flow cytometry; Human T lymphocyte ...
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electroporation apparatus 电穿孔仪 ; 细胞电转仪
Electroporation instruments 电激转化仪
electroporation transformation 电激法
irreversible electroporation 不可逆电穿孔 ; 不可逆电穿透技术
Electroporation and Electrofusion 电穿孔融合仪
Electroporation therapy 电穿孔疗法
Electroporation Cuvettes 细胞电击管
Electroporation system 细胞融合仪
Electroporation is the most effective non-viral technique for exogenous gene transfer, and it has been applied extensively due to its convenience and high efficiency.
电穿孔基因转移技术是外源基因转移最为有效的一种非病毒载体法,其突出的优点是简便、高效,已广泛用于体外基因转移。
参考来源 - 原位眼内恶性肿瘤模型生物学行为及抗新生血管生成基因治疗研究Therefore, applications of the electroporation transdermal drug delivery technology in clinical has great potential and good prospect.
因此将电致孔经皮给药技术应用于临床,具有巨大的潜力与良好的应用前景。
参考来源 - 电致孔条件下苦参经皮给药的研究Methods A signal peptide sequence(sp) was synthesized and cloned into the integrative plasmid pIlac down-stream of lactose-inducible promoter. The recombined plasmid pIlac-sp-IL10 was then introduced into Lactobacillus casei(L. casei)CECT 5276 by electroporation.
方法人工合成信号肽系列后与IL-10基因连接后克隆到干酪乳酸杆菌(Lactobacillus casei,L.casei)整合型表达载体pIlac的lac启动子下游,得到重组质粒pIlac-sp-IL10,电穿孔转化L.caseiCECT 5276。
参考来源 - 构建表达ILTherefore maintaining the osmotic pressure may be an effective way to improve the rate of electroporation.
在电击转化过程中,维持细胞渗透压是提高转化效率的有效方法。
参考来源 - 杜氏盐藻GFP表达载体的构建及电转化The result of sequencing showed that the insert gene could encode SOD correctly; After successfully transforming pMG36e-sod into L6032 by electroporation the activity of SOD was about 590.5NU∕mg prot.
核酸测序显示插入的sodA片段能正确编码SOD氨基酸序列。 利用电转化成功地将重组质粒导入L6032中,SOD的表达活性约590.5NU/mgprot。
参考来源 - 锰超氧化物歧化酶基因的克隆和在保加利亚乳杆菌中的表达The results showed that electroporation can not bring on an obvious enhancement of transformation efficiency.
以期能够获得高效转化最佳条件,结果发现电击转化并不能成倍提高转化效率。
参考来源 - 稻瘟病菌Magnaporthe oryzae高效转化文库的构建To simplify the approach, we replaced the electroporation method by heat-shock method for E. coli transformation and eliminated the procedures as dialysis etc.
同时,用热激法替代了电击法进行质粒的大肠杆菌转化,并去掉了透析等步骤,简化了构建过程。
参考来源 - BADH基因转化107杨及构建甜菜碱合成相关酶多基因表达载体Major methods for genetransformation include Agrobacterium mediated, PEG untake, electroporation,particle gun, microinjection, laser microbeam, pollen-tube pathway, ect. . Genetransformation mediated by Agrobacterium is widely used in Brassica breeding.
基因工程技术主要包括农杆菌介导转化法,PEG原生质体融合法,电激法,基因枪法,显微注射法,花粉管通道法等。
参考来源 - 反义芥子酶基因转入甘蓝型油菜的研究·2,447,543篇论文数据,部分数据来源于NoteExpress
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Objective To optimize the transfection rate in COS 7 cell line with electroporation method.
目的以电穿孔法转染COS 7细胞系以得到较高的转染率。
Separately changes the pulse voltage and temperature in the study of yeast cells by electroporation.
分别改变脉冲电压和环境温度,研究了酵母细胞的电穿孔情况。
The correct plasmid was linearized and transformed into Pichia Pastoris strain GS115 by electroporation.
将正确连接的表达质粒线性化后用电穿孔法转化到毕赤酵母GS115中。
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