Electrical pulse; the distribution of electric field; electroporation.
电脉冲；电场分布；电穿孔。
The recombinant plasmid was transfected into the cell line HL-60 by electroporation.
采用电转染方法将重组体导入HL-60细胞中；
Objective To optimize the transfection rate in COS 7 cell line with electroporation method.
目的以电穿孔法转染COS7细胞系以得到较高的转染率。
In this paper, we will report a new approach for the treatment of tumor-electroporation therapy.
本文采用一种新的方法—电穿孔结合化学药物进行肿瘤治疗的电化学疗法。
Separately changes the pulse voltage and temperature in the study of yeast cells by electroporation.
分别改变脉冲电压和环境温度，研究了酵母细胞的电穿孔情况。
The correct plasmid was linearized and transformed into Pichia Pastoris strain GS115 by electroporation.
将正确连接的表达质粒线性化后用电穿孔法转化到毕赤酵母GS115中。
We use two different dsRNA deliveries and the result indicated that electroporation was better than soaking.
比较了不同的转染方式对靶基因表达的干扰效果，结果证明电击法的效果略优于浸泡法。
The genetic transformation of basidiomycetes was carried out with PEG and electroporation and microprejection.
担子菌遗传转化目前主要采用电激法和PEG法。
The mice and chickens were immunized twice, 3 weeks apart, by electroporation into muscles or intramuscular injection.
小鼠和鸡分别经电穿孔和肌肉注射免疫两次，间隔为3周。
OBJECTIVE To study the effects of electroporation on the permeation of small molecular ion drug through skin in vitro.
目的研究电穿孔技术对小分子离子型药物经皮渗透的影响。
Accordingly, the electroporation can enhance the cytotoxicity of antitumor drug. This is a piece of new approach for antitumor.
结果说明，利用电穿孔可提高抗肿瘤药物的细胞毒性，为治疗肿瘤提供了新的途径。
AIM to study the enhancing effect of electroporation and iontophoresis on the permeation of insulin through human cadaver skin in vitro.
目的利用离体皮肤渗透技术研究了熊果苷柔性脂质体的经皮渗透和几种渗透促进剂对其影响。
Methods a retroviral vector containing TK gene was constructed and transduced into pulmonary adenocarcinoma cell A549 by electroporation.
方法构建含tK基因的逆转录病毒表达载体，电穿孔法转化肺癌细胞A549。
Objective to investigate the effects of erythropoietin (EPO) gene transfer into skeleton muscle mediated by electroporation on renal anemia.
目的研究电脉冲介导的质粒红细胞生成素(EPO)基因肌肉转移效率以及对肾性贫血的治疗作用。
OBJECTIVE To investigate the entrapment of insulin in skin via electroporation, diminish the entrapment and enhance the transdermal delivery of insulin.
目的研究和克服胰岛素在电致孔下经皮传输过程中在皮肤中的滞留，提高胰岛素的经皮渗透速率。
These results suggest that cataplasma to combine with electroporation may be an efficient alternative for transdermal drug delivery of hydrophilic molecules.
结论：瞬间脉冲电场能够显著提高水凝胶贴剂的透皮给药效果，两者结合将成为一种新型透皮给药技术。
Electroporation refers to the use of electro-osmosis and the principle of non-invasive painless to import active ingredients or nutrition to beauty therapy skin.
是指利用电穿孔和电渗透的原理，无创无痛的把活性成份或营养导入到皮下的美容疗法。
Objective To optimize the electroporation parameters in mouse primary lymphocyte cultured in suspension with adenosine deaminase acting on RNA 1(ADAR1) as a reporter gene.
目的以RNA依赖性腺嘌呤脱氨酶（ADAR1）为靶基因优化悬浮培养的小鼠原代淋巴细胞的电穿孔转染条件。
Objective to study the effect of electric field direction on the percutaneous penetration of the non-ionic drug caffeine mediated by skin electroporation and iontophoresis.
目的研究电穿孔及离子导入时电场方向对分子型药物咖啡因经皮渗透的影响。
OBJECTIVE To enhance the transdermal delivery of lipophilic drugs through the local hydrophilic transport regions (LTRs) formed during electroporation by applying surfactant.
目的利用表面活性剂的促渗作用，提高脂溶性药物通过电致孔形成的水性通道的经皮渗透量。
This article mainly elaborated the superiority of electroporation in gene therapy and the influencing factors, as well as the applications in the preclinical and clinical trial.
本文重点阐述了电穿孔在基因治疗中的优势、影响因素及在临床前和临床试验中应用。
In the first section, that electroporation can be induced on chick's erythrocytes by high-intensity electric pulses was investigated. The most direct proof of morphology was acquired.
第一部分研究了强电脉冲可导致离体鸡血细胞产生电穿孔，获得了最直接的形态学证据，并对电穿孔产生的机理作了初步探讨。
For example, ED5 reduces cortical Egr-1 mRNA expression and interstitial fibrosis in rats 7 days after renal obstruction (unilateral ureteral ligation) and delivery by electroporation.
例如，ED5可以减少小鼠尿路梗阻（单侧输尿管结扎）或通过电穿孔法切除肾脏7天后皮质早期生长反应基因-1信使RNA表达和间质纤维化。
The direct gene transfer systems involved the chemical method (PEG), electroporation, microinjection, particle bombardment, pollen-mediated gene transfer and other systems using various vectors.
阐述了化学导入法(PEG)、电穿孔法、注射法，粒子轰击法、花粉管萌导法及使用其它载体系统的成功实例。
The direct gene transfer systems involved the chemical method (PEG), electroporation, microinjection, particle bombardment, pollen-mediated gene transfer and other systems using various vectors.
阐述了化学导入法(PEG)、电穿孔法、注射法，粒子轰击法、花粉管萌导法及使用其它载体系统的成功实例。
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