Ten newly born SD rats of clean grade were selected to isolate the brain tissue under sterile condition and clone brain-derived NSCs.

实验选取清洁级SD新生鼠10只，无菌条件下分离脑组织，克隆传代培养脑源性神经干细胞。

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Kunming mice of clean grade were purchased from animal Experiment Center, Nanjing University of Traditional Chinese Medicine (number of animal license SYXK 2005-0009).

清洁级昆明种小鼠购自南京中医药大学实验动物中心(动物生产许可证号为SY XK(苏)2005 - 0009)。

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Methods 24 rats of clean grade were randomly divided into 3 groups: acyl ethylene dimethyl glycine (TMOG) group and the control group, sham-operated group, 8 in each group.

方法健康清洁级大鼠24只，随机分为3组：二甲基乙二酰基甘氨酸（TMOG）组、对照组、假手术组，每组8只。

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