Ten newly born SD rats of clean grade were selected to isolate the brain tissue under sterile condition and clone brain-derived NSCs.
实验选取清洁级SD新生鼠10只,无菌条件下分离脑组织,克隆传代培养脑源性神经干细胞。
Kunming mice of clean grade were purchased from animal Experiment Center, Nanjing University of Traditional Chinese Medicine (number of animal license SYXK 2005-0009).
清洁级昆明种小鼠购自南京中医药大学实验动物中心(动物生产许可证号为SY XK(苏)2005 - 0009)。
Using SPF(Specific pathogen free)animals as the breeding stock and adopting synthesize preventive measure, the experiment mouse and rat of clean grade were reared in barrier system for 2 years.
以SPF级(无特殊病原体)动物为种源,采取综合防治措施,在屏障设施饲养清洁级实验小鼠和大鼠。
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