Ten newly born SD rats of clean grade were selected to isolate the brain tissue under sterile condition and clone brain-derived NSCs.

实验选取清洁级SD新生鼠10只，无菌条件下分离脑组织，克隆传代培养脑源性神经干细胞。

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Kunming mice of clean grade were purchased from animal Experiment Center, Nanjing University of Traditional Chinese Medicine (number of animal license SYXK 2005-0009).

清洁级昆明种小鼠购自南京中医药大学实验动物中心(动物生产许可证号为SYXK(苏)2005- 0009)。

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Using SPF(Specific pathogen free)animals as the breeding stock and adopting synthesize preventive measure, the experiment mouse and rat of clean grade were reared in barrier system for 2 years.

以SPF级（无特殊病原体）动物为种源，采取综合防治措施，在屏障设施饲养清洁级实验小鼠和大鼠。

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Using SPF(Specific pathogen free)animals as the breeding stock and adopting synthesize preventive measure, the experiment mouse and rat of clean grade were reared in barrier system for 2 years.

以SPF级（无特殊病原体）动物为种源，采取综合防治措施，在屏障设施饲养清洁级实验小鼠和大鼠。

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