An amplified immunoassay with improved sensitivity has been proposed based on two-step amplification procedures of enzyme-catalyzed precipitation and biotin-streptavidin binding system (in Chapter 3).
在用于实际试样的回收率测定中,结果良好。(2)提出了一种基于酶催化沉积底物和生物素-亲和素两步放大的超灵敏免疫检测方法(第3章)。
Streptavidin has a remarkably high affinity for its natural ligand, biotin.
链霉亲和素与它的配体具有显著的高亲和力。
The complex and irregular structure of the biotin-binding site makes it highly optimized for biotin binding and confers great specificity to the streptavidin-biotin complexes (4).
生物素结合位点的复杂性和结构的不规则性使得它最适合与生物素结合并且表现出与链霉素亲和素-生物素复合体的高度特异性(4)。
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