An amplified immunoassay with improved sensitivity has been proposed based on two-step amplification procedures of enzyme-catalyzed precipitation and biotin-streptavidin binding system (in Chapter 3).
在用于实际试样的回收率测定中，结果良好。(2)提出了一种基于酶催化沉积底物和生物素-亲和素两步放大的超灵敏免疫检测方法(第3章)。
Streptavidin has a remarkably high affinity for its natural ligand, biotin.
链霉亲和素与它的配体具有显著的高亲和力。
The complex and irregular structure of the biotin-binding site makes it highly optimized for biotin binding and confers great specificity to the streptavidin-biotin complexes (4).
生物素结合位点的复杂性和结构的不规则性使得它最适合与生物素结合并且表现出与链霉素亲和素-生物素复合体的高度特异性(4)。
Because of its strong non-covalent interaction with biotin, streptavidin can be used to isolate biotinylated proteins (1,2).
因为它的强高亲和力非共价结合，链霉素亲和素可以用于检测和分离生物素化的蛋白（1.2）。
Streptavidin is a 53,000 dalton tetrameric protein purified from the bacterium Streptomyces avidinii (3). Each subunit binds to biotin with extremely high affinity.
链霉素亲和素是从链霉亲生物素蛋白中分离出来的53，000道尔顿的同源四聚体（3），每一个链霉素亲和素的亚基可以与维生素形成高亲和力非共价结合。
The receptive group can be an antibody, a fragment of an antibody, an avidin, a streptavidin, or a biotin moiety.
该接受基团可以是抗体、抗体片段、抗生物素蛋白、链霉抗生物素蛋白、或生物素基序。
In addition, streptavidin coupled microsphere solution is introduced to to enlarge detection signal of the biotin-linked antibody.
此外，链霉抗生物素蛋白的耦合微球溶液被引入，以放大的生物素联型抗体的检测信号。
The magnetic nanoparticle probe was prepared by specifically connecting the streptavidin-conjugated magnetic nanoparticles and the antibody of analyte via the strong streptavidin-biotin interaction.
采用链霉亲和素包被磁纳米粒子，将生物素标记的特异性抗体偶联在磁纳米粒子上，制备出高特异性的磁纳米探针；
Method 4 kinds of block buffer, the different concentration of anti-MIF and biotin-anti-MIF, the different dilution of streptavidin-HRP are used for titration of ELISA test.
方法采用方阵滴定法，比较4种封闭液、不同稀释度的捕获抗体、检测抗体和辣根过氧化酶标记链菌素对检测结果的影响，确定ELISA法的最佳实验条件。
Method 4 kinds of block buffer, the different concentration of anti-MIF and biotin-anti-MIF, the different dilution of streptavidin-HRP are used for titration of ELISA test.
方法采用方阵滴定法，比较4种封闭液、不同稀释度的捕获抗体、检测抗体和辣根过氧化酶标记链菌素对检测结果的影响，确定ELISA法的最佳实验条件。
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