目的：获得抗人视网膜母细胞瘤单克隆抗体轻链可变区基因。
Objective: to acquire the variable region gene of light chain of monoclonal antibody against human retinoblastoma.
轻链可变区和恒定区均包含保守的半胱氨酸，形成链内和链间二硫键。
The cysteine residues involved in the formation of disulfide bonds are completely conserved among fish IgL.
方法利用RT - PCR方法从杂交瘤细胞株2f 3中扩增出单克隆抗体重链可变区和轻链可变区基因。
Methods the genes were amplified from the heavy (VH) and light (VL) chain variable regions of hybridoma cell strain 2f3 by RT-PCR and identified by DNA sequencing.
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