目的：获得抗人视网膜母细胞瘤单克隆抗体轻链可变区基因。
Objective: to acquire the variable region gene of light chain of monoclonal antibody against human retinoblastoma.
方法利用RT - PCR方法从杂交瘤细胞株2f 3中扩增出单克隆抗体重链可变区和轻链可变区基因。
Methods the genes were amplified from the heavy (VH) and light (VL) chain variable regions of hybridoma cell strain 2f3 by RT-PCR and identified by DNA sequencing.
目的获取系列鼠抗人CD分子单抗的轻链及重链可变区基因并进行克隆和测序。
Objective to obtain the VH and VL variable region genes of murine McAbs to human CD molecule.
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