电泳采用中性毛细管涂层柱和4%线性聚丙烯酰胺凝胶电泳缓冲液,紫外检测仪检测。
The neutral coated capillary and 4% linear polyacrylamide gel buffer were used, and the wave length of ultraviolet detector was 254 nm.
方法:比较以往报道的各种低分子量蛋白的电泳分离方法,对电泳缓冲液系统、分离胶和浓缩胶的浓度、电泳程序等进行了优化。
Methods: Based on several reported SDS-PAGE systems for low molecular weight protein, the buffer systems, concentrations of separating gel and stacking gel, electrophoresis procedures were optimized.
方法:比较以往报道的各种低分子量蛋白的电泳分离方法,对电泳缓冲液系统、分离胶和浓缩胶的浓度、电泳程序等进行了优化。
Methods: Based on several reported SDS-PAGE systems for low molecular weight protein, the buffer systems, concentrations of separating gel and stacking gel, electrophoresis procedures were optimized.
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