从人胚胎生殖嵴、肠系膜中消化分离的原始生殖细胞，将其接种在人子宫内膜成纤维细胞饲养层上传代培养。
PGC from genital ridge and mesenterium of human embryo was incubated on fibroblast feeder layers for subculture.
方法：牛晶体上皮细胞原代培养。
Methods: Bovine lens epithelial cells (BLEC) were cultured in vitro.
第二部分，综述现代培训理论与方法。
Part II statement of contemporary training theory and method.
结果成功原代培养了猪眼rpe细胞。
Results Primary porcine RPE cells were cultured successfully.
结论：高温可诱发原代培养神经细胞凋亡。
Conclusions: Hyperthermia may induce apoptosis of neuroepithelium in primary culture.
目的：探索人脂肪源性基质细胞原代培养方法。
Objective: To establish a primary human adipose-derived stromal cells culture method.
目的探索人心房肌细胞的原代及传代培养方法。
Objective To explore a method for primary culture and subculture of human atrial myocardial cells.
目的探讨体外原代培养大鼠肝细胞的分离方法。
ObjectiveTo explore the isolating methods of rat hepatocytes for primary culture.
试管苗再生能力随继代培养代数的增加而提高；
The more the subculture times were, the higher the regeneration capability was.
目的：探索原代培养大鼠肝细胞的最佳分离方法。
Aim: To explore the best isolation methods of rat liver cells from primary culture.
方法以无血清培养基体外原代培养角质形成细胞。
Methods Human keratinocytes were primarily cultured in vitro with serum free medium.
目的对大肠腺瘤细胞原代培养的方法作进一步的改进。
Objective To improve the primary cell culture methods of colorectal adenomas.
因此，存在许多与这些目标相关的传统和现代培训形式。
As a consequence, a multitude of training forms exist, both traditional and modern, which correspond to those aims.
二是先诱导获得愈伤组织，再经继代培养获得胚胎发生。
Embryogenic callus and somatic embryos could be directly obtained on the medium only with ZT, but it was influenced by explant sources.
用组织块直接培养法，也可以得到皮肤的原代培养细胞。
The primary culture cells of rabbit skin tissue could be obtained by explant culture too.
结论添加晶体皮质可提高晶体上皮细胞原代培养成功率。
ConclusionAdding lens cortex can increase the success rate of primary culture of lens epithelial cells.
迄今为止我们已取得了突变的原代培养的细胞以及蠕虫”。
To date we have made mutant tissue-cultured cells as well as worms.
微格教学是现代培养专业教师基本教学技能的一种先进方法。
Microteaching is a kind of advanced way to develop professional teachers' skills in modern times.
周村商埠文化的形成主要有两个渠道：传统继承和现代培植。
Zhoucun trading ports culture has two main channels: inheritance of the traditional and modern cultivation.
说明原代培养细胞在血清中能分化为神经元及神经胶质细胞。
It demonstrated that some of the differentiated cells are neurons and the others were glial cells.
目的探讨淫羊藿甙对体外原代培养神经元缺氧缺糖损伤的作用。
Objective To investigate the effects of Icariin on anoxic and glucose-deprived injury in primary cultured neurons.
结论：酶消化法原代培养牛视网膜周细胞是一种稳定、可靠的方法。
Conclusion The enzyme digestion procedure is a stable and reliable method to obtain bovine retinal pericytes.
经过两年多(15个世代)的继代培养，建立了党参体细胞无性系。
Somatic clones were established after 15 generations of subculture for more than two years.
目的研究原代培养的脊髓神经干细胞在小鼠视网膜的整合和分化情况。
Objective to study the integration and differentiation of spinal neural stem cells after transplantation into mice retina.
方法：膜片钳全细胞记录测量原代培养大鼠海马神经元膜的钾离子电流。
Methods: The potassium currents of rat hippocampal neurons in primary culture were measured with patch clamp whole cell recording.
研究芳烃类有机溶剂对原代培养大鼠神经胶质细胞的谷氨酸重摄取的影响。
To study effect of aromatic hydrocarbon solvents on the reuptake of amino acid by primary cultured glia cells.
从水稻成熟胚诱导出的愈伤组织经继代培养后可以产生大量的胚性愈伤组织。
Many embryogenic calli were produced by subculture of calli induced from mature embryo of rice.
结果：原代培养的RPE细胞含大量色素呈多角形，传代的细胞透明呈梭形。
Results: Primary cultured RPE cells contained large amount of pigments in polygonal shape. Passages of cultured RPE cells presented transparent spindle shape.
背景：人胚胎干细胞传代培养的关键是抑制其自发分化、保证细胞的全能性。
BACKGROUND: Key point for subculture of human embryonic stem cells is to inhibit spontaneous differentiation and make sure totipotency of cells.
兔角膜上皮和内皮细胞组织块原代培养，消化接种于人胚肺饲细胞瓶传代培养。
After primary culture, rabbit corneal epithelium and endothelium were digested and inoculated in bottle having hFLP feeder cells for serial passage.
应用推荐