从人胚胎生殖嵴、肠系膜中消化分离的原始生殖细胞，将其接种在人子宫内膜成纤维细胞饲养层上传代培养。
PGC from genital ridge and mesenterium of human embryo was incubated on fibroblast feeder layers for subculture.
用好现代培训方法，不断提升教员教学能力。
Take good use of modern training methods and improve the teaching ability of teachers.
微格教学是现代培养专业教师基本教学技能的一种先进方法。
Microteaching is a kind of advanced way to develop professional teachers' skills in modern times.
在植物组织培养过程中，继代培养是其中的重要环节，而长期继代则是种质资源离体保存的必要手段。
Subculture is an important link in the process of the tissue culture and the long-term subculture is the essential method for conservation in vitro of germplasm.
自2005年以来，蔚蓝国际就致力于创建全球性的模拟教育平台，以帮助中国年轻一代培养国际视野，领导能力和综合素质。
Since 2005, WEMUN has dedicated itself to building an international educational platform, training students to have an international outlook and refine their leadership skills.
方法：牛晶体上皮细胞原代培养。
Methods: Bovine lens epithelial cells (BLEC) were cultured in vitro.
结果成功原代培养了猪眼rpe细胞。
Results Primary porcine RPE cells were cultured successfully.
结论：高温可诱发原代培养神经细胞凋亡。
Conclusions: Hyperthermia may induce apoptosis of neuroepithelium in primary culture.
目的：探索人脂肪源性基质细胞原代培养方法。
Objective: To establish a primary human adipose-derived stromal cells culture method.
目的探索人心房肌细胞的原代及传代培养方法。
Objective To explore a method for primary culture and subculture of human atrial myocardial cells.
目的探讨体外原代培养大鼠肝细胞的分离方法。
ObjectiveTo explore the isolating methods of rat hepatocytes for primary culture.
经3～4次继代培养即可得到悬浮的单细胞。
Suspension single cells were obtained after successive subculturing of 3 or 4 generations.
目的：探索原代培养大鼠肝细胞的最佳分离方法。
Aim: To explore the best isolation methods of rat liver cells from primary culture.
方法以无血清培养基体外原代培养角质形成细胞。
Methods Human keratinocytes were primarily cultured in vitro with serum free medium.
本研究拟建立人嗜铬细胞瘤细胞的原代培养方法。
The study is to establish a method of separating and primarily culturing of human pheochromocytoma cells.
目的对大肠腺瘤细胞原代培养的方法作进一步的改进。
Objective To improve the primary cell culture methods of colorectal adenomas.
因此，存在许多与这些目标相关的传统和现代培训形式。
As a consequence, a multitude of training forms exist, both traditional and modern, which correspond to those aims.
结论添加晶体皮质可提高晶体上皮细胞原代培养成功率。
ConclusionAdding lens cortex can increase the success rate of primary culture of lens epithelial cells.
探讨脑活素对原代培养大鼠大脑皮质神经元的作用及机制。
To investigate the effect of cerebrolysin on primary cultured rat cortical neurons and its mechanisms of action.
周村商埠文化的形成主要有两个渠道：传统继承和现代培植。
Zhoucun trading ports culture has two main channels: inheritance of the traditional and modern cultivation.
说明原代培养细胞在血清中能分化为神经元及神经胶质细胞。
It demonstrated that some of the differentiated cells are neurons and the others were glial cells.
目的探讨淫羊藿甙对体外原代培养神经元缺氧缺糖损伤的作用。
Objective To investigate the effects of Icariin on anoxic and glucose-deprived injury in primary cultured neurons.
目的：探讨温脾汤药物血清对原代培养大鼠系膜细胞增殖的影响。
Objective:To study the effect of Wenpitang pharmacologic serum on proliferation of mesangial cells of rats in vitro.
结论：酶消化法原代培养牛视网膜周细胞是一种稳定、可靠的方法。
Conclusion The enzyme digestion procedure is a stable and reliable method to obtain bovine retinal pericytes.
经过两年多(15个世代)的继代培养，建立了党参体细胞无性系。
Somatic clones were established after 15 generations of subculture for more than two years.
目的研究原代培养的脊髓神经干细胞在小鼠视网膜的整合和分化情况。
Objective to study the integration and differentiation of spinal neural stem cells after transplantation into mice retina.
本文探讨了连续传代培养中营养对昆虫病原线虫繁殖力及产量的影响。
The effects of nutrition on fecundity and yield of nematode in continued culture were evaluated.
目的研究氟对原代培养大鼠海马神经元氧化应激和DNA损伤的影响。
Objective To explore the effects of fluoride on oxidative stress and DNA damage in primary hippocampal neurons of rats.
方法：膜片钳全细胞记录测量原代培养大鼠海马神经元膜的钾离子电流。
Methods: The potassium currents of rat hippocampal neurons in primary culture were measured with patch clamp whole cell recording.
目的：建立一种简单、较理想的新生大鼠皮层神经元体外原代培养方法。
Objective: to establish an easier and better primary culture technique suitable to neurons of newborn rat cortical tis-sure.
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