锌指核酸酶被设计识别基因组特定DNA序列，之后对其进行酶切，进而删除或替换这段序列。
Zinc-finger nucleases are enzymes that can be designed to find specific DNA sequences in the genome and cut them out, deleting those sequences or swapping one gene for another.
通过酶切、PCR及测序鉴定各重组体。
The recombinants were analyzed and identified by restriction enzyme, PCR and sequencing.
用酶切和PCR的方法对重组质粒进行鉴定。
Used restrict enzyme and PCR to verify the reconstructed plasmid.
方法：采用DNA测序及限制性酶切反应方法。
Methods:DNA sequencing and restriction endonuclease reaction was used.
乐复能以胰蛋白酶酶切后，用HPLC分析肽图；
The peptide map of novaferon was obtained by trypsin digestion and HPLC assay.
同时对其纯度、浓度、分子量及酶切图谱进行了鉴定和分析。
Meanwhile, we also studied its purity, molecular weight, concentration and restrictive enzymatic map.
重组质粒经酶切和测序证实正确，然后转化大肠杆菌BL21。
The recombinant plasmid was confirmed by digestion and sequence, then transformed into E. coli BL21.
本研究利用多种组合的酶切分析，绘制了较为详细的酶切图谱。
In this study, a detailed restriction map was constructed using multiple groups of restriction endonuclease analysis.
结果酶切和测序证实PTEN基因克隆和真核表达载体构建成功。
Results Restriction enzyme analysis and DNA sequence analysis showed that PTEN gene was cloned and the eukaryotic expression vector was constructed successfully.
结果酶切及测序显示对OSCP基因有义突变部分的纠正获得成功。
Result Restriction endonuclease digestion and sequencing showed that the modification of the sense mutation of OSCP gene can be successful.
结果经酶切和DNA测序证明LIF基因序列正确，载体构建成功。
Results: LIF gene sequence and vector were verified correctly by enzyme digestion, sequence analysis.
用8%非变性聚丙烯酰胺凝胶电泳将酶切产物分离，并用银染法显色。
The products of endonuclease digestion were run on 8% non-denaturing polyacrylamide gel electrophoresis and detected by silver staining.
外源基因在细胞内的酶切保护问题是影响基因转染成败的重要因素之一。
Protection of exogenous genes against intracellular conditions is one of key factors in gene transfection.
限制性酶切图谱分析证实这两个基因的结构是完整的，符合转基因实验要求。
Plasmid DNAs were prepared by alkali lysis and purified with polyethylene glycol 8000. Restriction enzyme analysis show that both genes are well-constructed suitable for transgenic animal experiment.
结果：经PCR，酶切及测序方法鉴定，该腺病毒-整合酶嵌合系统构建成功。
Results:After being identified by PCR, restriction enzyme digestion and sequencing, the adeno-integrase hybrid system was successfully constructed.
建立和优化了大麦dna单限制性酶切选择性扩增多态性技术体系(SADF)。
A method named selective amplification DNA fragments (SADF) using single restrictive enzyme was established and optimized in barley.
根据所得的酶切类型，计算了种间每核苷酸位点的平均碱基取代值P（遗传距离）。
We estimated the average number of nucleotide substitutions per site (genetic distance, P) among these species according to the cleavage patterns and Nei & Li's models(1979).
结果经酶切和测序鉴定表明所构建的重组表达质粒中含有TSO45 - 4b基因。
Results: the constructed recombinant plasmid contained the sequence of TSO45-4B gene that was identified by endonuclease digestion and sequence analysis.
结果酶切鉴定和DNA测序分析显示，TROP2靶向RNA干扰重组质粒构建成功。
Results Enzyme digestion analysis and DNA sequencing showed that TROP2 targeted RNA interference recombinant plasmids were constructed successfully.
结论：经酶切鉴定构建的人透明带蛋白3重组表达载体可高效表达重组人zp3蛋白。
Conclusion The recombinant expression plasmid constructed by restriction enzyme cleave identification can highly express recombinant human ZP3 protein.
PCR检测和酶切鉴定结果表明，在水平测试的混合饲料粉中可检出牛源、羊源性成分。
The bovine, sheep and goat derived materials were positive in mixed feed powders of level test by PCR and restriction enzyme analysis.
结果构建的EBO G87和EBO WT重组载体经内切酶双酶切鉴定及核苷酸序列测定证实。
Results The constructed vectors of EBO-WT and EBO-G87 were identified by restriction enzyme digestion and nucleotide sequencing.
目的人鼻病毒3c蛋白酶具有酶切物异性强且在低温下仍保持较强酶活性的特点而被用作工具酶。
Objective Application of human rhinovirus 14 3c protease as a tool-enzyme for more stringent sequence specificity and efficient cleavage at low temperature.
同时在连续传代40代后每隔5代提取重组病毒全基因组进行酶切鉴定，发现其酶切位点保持不变。
To evaluate its heredity stability, the recombinant virus genome was extracted and digested by Hindlll every 5 generations, and 40 generations later, its enzyme digestion sites almost keep stable.
通过对内切酶用量、酶切时间、连接时间、稀释倍数等的研究，建立了适于核桃分析的AFLP银染技术体系。
After systematically study on the main factors involved, an AFLP silver-staining analysis system suitable for walnut genomic DNA was established.
结论：由于PCR -双酶切方法快速、简单、易操作，且特异性好，可作为CMT1A基因诊断的一种初筛方法。
CONCLUSION: Because of the speediness, simpleness and good specificity, the PCR combined with restriction enzyme digestion can be used as a primary screening in the gene diagnosis of CMT1A.
方法：应用聚合酶链反应（PCR）-双酶切法，对23例CMT1患者和30例正常人进行基因特异性连接片段的检测。
METHODS:Polymerase chain reaction(PCR) combined with restriction enzyme digest ion were used to detect gene specific junction fragments of the 23 CMT1 patients and 30 normal controls.
用9个玉米和水稻线粒体基因探针与酶切条带杂交发现，不育系与保持系线粒体DNA的相同大小酶切片段含有相似的顺序。
Homologous sequences of mtDNA were found between MS and maintainer lines by using 9 probes of maize and rice to hybridize with digested mtDNA.
结果经pcr方法、酶切鉴定及直接测序鉴定出YMDD、YVDD和YIDD质粒构建成功，且该方法具有较好的敏感性和特异性。
Results PCR, restriction endonuclease method and direct sequence analysis demonstrated that plasmids of YMDD, YVDD and YIDD were constructed successfully.
结果经pcr方法、酶切鉴定及直接测序鉴定出YMDD、YVDD和YIDD质粒构建成功，且该方法具有较好的敏感性和特异性。
Results PCR, restriction endonuclease method and direct sequence analysis demonstrated that plasmids of YMDD, YVDD and YIDD were constructed successfully.
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