目的改良大鼠胃黏膜壁细胞分离方法。
Objective To improve the means to isolate gastric parietal cells in rats.
方法：细胞分离和培养。
旋毛虫的体细胞分离，可以不用加胰酶消化。
It need not add pancreatin to separate the somatic cells of Trichinella spiralis.
使用淋巴细胞分离液和3%的明胶分离胎儿细胞。
Fetal cells were isolated using lymphocyte separation liquid and 3% gelatin.
目的建立有效的人胎儿神经前体细胞分离及纯化系统。
Objective To establish an effective system of isolating and purifying neural precursors from human fetal brain.
悬浮培养8天左右的玫瑰茄细胞最适宜于单细胞分离；
The cell suspension cultures for 8days were most favourable for the formation of cell clones.
目的：建立稳定的人脐静脉内皮细胞分离和培养的方法。
AIM: to establish a stable method to isolate and culture human umbilical vein endothelial cells in vitro.
目的：探索一种简便、高效的脐血单个核细胞分离方法。
Objective: To explore an convenient and high-performance isolation method of mononuclear cells derived from human cord blood.
本文主要对人类EG细胞分离及体外培养的体系进行优化。
This article focuses mainly on optimization of human EG cell isolation and in vitro culture system.
目的：探索和建立大鼠肠神经干细胞分离与体外培养的方法。
Objective: To explore and establish the methods of isolation and culture in vitro of rats enteric neural stem cells.
目的优化胰岛细胞分离纯化的方法，提高胰岛产量、纯度和功能。
Objective To improve output, purification and function of islet cells by optimizing isolation and purification methods.
方法改进成肌细胞分离方法，即缩短分离时间并增加分离细胞数。
Methods the previous method to isolate myoblasts was modified by reducing the cell isolating time and increasing the number of isolated cells.
通过鸡胚和鸡胚细胞分离得到Q株，为鸡传染性法氏囊病病毒株。
Virus strains Q were isolated through chicken embryo and chicken embryo cell culture.
表皮细胞分离培养技术的第一步就是要从皮肤中分离基底层细胞。
Isolation and culture of Keratinocyte, it is the first step to isolate the epidermal basal layer cells.
人胚胎成纤维细胞饲养层在ES细胞分离过程中具有较好的作用。
Human embryonic fibroblast feeder played a positive role during the ES cells isolation procedure.
表明SD大鼠心肌细胞条件培养基可用于兔胚胎干细胞分离培养。
The essay showed SD rat cardiomyocyte conditioned medium can be used to isolation and culture of rabbit ES-like cell.
结论在本实验室，离心淘洗技术已能成功应用于肝脏细胞分离过程。
Conclusion Centrifugal elutriation technique could be applied successfully during the separation of liver cells in our laboratory.
在小鼠身上的研究表明，运动开启遗传计划导致心脏生长，心肌细胞分离。
The studies in mice suggest that exercise turns on a genetic program that leads the heart to grow as heart muscle cells divide.
实验结果表明算法对菌群聚堆细胞分离的成功率要高于现有算法10%以上。
The experiment results show the success rat IO to separate bacteria touching-cell is 10% higher than the existing algorithms.
失巢凋亡是一种当细胞与其周围的基质和邻近细胞分离后而被启动死亡的过程。
Anoikis is the process by which cells are triggered to die when separated from their surrounding matrix and neighboring cells.
结论：白细胞分离术可有效减轻白血病的细胞负荷，避免肿瘤细胞溶解综合征；
Conclusion:The leukapheresis could decrease effectively tumor loads and leukostatic sludging without acute tumor lysis syndrome.
为进行神经生物学和神经药理学的研究，我们建立了胚胎脑细胞分离培养的方法。
In order to study neurobiology and neuropharmacology, dissociated culture method of brain cells were established.
这是首次比较全面的揭示了细胞分离导致人细胞凋亡的机制，被刊登在干细胞杂志上。
Reported in the journal cell Stem cell, these results provide a first ever comprehensive elucidation of mechanisms underlying dissociation-induced apoptosis in human es cells.
传统的亚细胞分离技术结合质谱鉴定技术为亚细胞蛋白质组学的研究提供了技术平台。
The classical subcellular fractionation methods coupled with mass spectrometry identification technique have created opportunities for the study of subcellular fractions.
目的：为深入探讨肝纤维化的细胞机制，探索经济、稳定的肝星状细胞分离和培养方法。
Objective: To investigate the method of isolation and culture of hepatic stellate cells for studying the cellular mechanisms of hepatic fibrosis.
基于微过滤原理设计的闸式细胞分离芯片，可实现老鼠外周血中白细胞与红细胞的分离。
Based on the principle of microfilter, gate-type cell sorter chip was designed, which can separate the white blood cells from the rat peripheral blood.
无论在室温中放置多久，细胞系都不会粘附于底物，因此应在细胞分离后进行活体培养。
The cell line does not adhere to the substrate when left at room temperature for any length of time, therefore, live cultures may be received with the cells detached.
方法：细胞分离培养于椎板和髂嵴的松质骨，镜下计数细胞数目和台盼蓝染色检测细胞活力。
Methods. Cells obtained from cancellous bone of both vertebral lamina and iliac crest were cultured and proliferation was evaluated by direct cell counting and viability detected by Trypan blue.
方法：细胞分离培养于椎板和髂嵴的松质骨，镜下计数细胞数目和台盼蓝染色检测细胞活力。
Methods. Cells obtained from cancellous bone of both vertebral lamina and iliac crest were cultured and proliferation was evaluated by direct cell counting and viability detected by Trypan blue.
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