Recombinant clone can be analyzed directly with the use of PCR. It is very efficient, especially in establishment of subtractive library of cDNA.
筛选重组阳性克隆可直接用细菌悬液作PCR模板，在消减文库构建时，能大大提高工作效率。
Results and Conclusion: the PCR screening method was convenient and fast for confirming positive recombinant clone, and there was no need for preparation and purification of the plasmid.
结果和结论：以pcr方法筛查重组阳性克隆，可以简便快速鉴定重组阳性克隆，不需提取质粒。
Clone and express bradyzoite antigen 1(BAG1) gene of T. gondii, and analyze the immunoreactivity of the recombinant product.
克隆与表达弓形虫缓殖子期特异性抗原1（BAG1）的基因，并分析重组抗原的免疫反应性。
Objective: To clone and express recombinant HSV-2 antigen in E. coli.
目的：在大肠杆菌中表达重组单纯疱疹病毒2型(HSV - 2)抗原。
Objective To clone and express bradyzoite antigen 1(BAG1) gene of T. gondii, and analyze the immunoreactivity of the recombinant product.
目的克隆与表达弓形虫缓殖子期特异性抗原1（BAG1）的基因，并分析重组抗原的免疫反应性。
Objective to clone and construct the plasmid containing human thyroid stimulating hormone receptor (TSHR) gene ectodomain, and then identify the immunoreactivity of the purified recombinant protein.
目的克隆人促甲状腺激素受体胞外段基因，构建重组真核表达质粒，获得具有免疫学活性的纯化重组蛋白。
Objective To construct the recombinant plasmid that highly expressed human zona pellucida 3. Methods The techniques of PCR amplification, T-A vector ligation, and sub-clone were used.
目的：构建高效表达人透明带蛋白3的真核重组表达载体。方法：利用PCR、T- A载体克隆和亚克隆等技术。
Objective To clone human ornithine decarboxylase antizyme (OAZ) mutation gene and express its recombinant protein.
目的构建人鸟氨酸脱羧酶抗酶(OAZ)突变基因，重组至原核表达载体中并表达出重组蛋白。
Objective To clone human ornithine decarboxylase antizyme (OAZ) mutation gene and express its recombinant protein.
目的构建人鸟氨酸脱羧酶抗酶(OAZ)突变基因，重组至原核表达载体中并表达出重组蛋白。
应用推荐