Recombinant clone can be analyzed directly with the use of PCR. It is very efficient, especially in establishment of subtractive library of cDNA.
筛选重组阳性克隆可直接用细菌悬液作PCR模板，在消减文库构建时，能大大提高工作效率。
Results and Conclusion: the PCR screening method was convenient and fast for confirming positive recombinant clone, and there was no need for preparation and purification of the plasmid.
结果和结论：以pcr方法筛查重组阳性克隆，可以简便快速鉴定重组阳性克隆，不需提取质粒。
Clone and express bradyzoite antigen 1(BAG1) gene of T. gondii, and analyze the immunoreactivity of the recombinant product.
克隆与表达弓形虫缓殖子期特异性抗原1（BAG1）的基因，并分析重组抗原的免疫反应性。
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