The sodium currents were detected by patch-clamp technique in whole-cell recording mode.
用膜片钳技术的全细胞模式测定全细胞钠电流并观察应用特布他林对它的影响。
Method The experiment was carried out in freshly isolated bullfrog using a whole-cell patch-clamp technique.
方法实验采用的方法是全细胞膜片钳技术，实验材料为急性分离的牛蛙背根神经节神经元。
Methods In acutely isolated rat DRG neurons, effects of loureirin B on capsaicin-evoked currents were observed using whole-cell patch-clamp technique.
方法采用全细胞膜片钳技术在急性分离的大鼠背根神经节细胞上观察龙血素B对辣椒素诱发的辣椒素受体电流的影响。
METHODS: A tight seal whole-cell patch-clamp technique was used to record ITO, IK, and IK1 in single cells isolated from guinea pig and rabbit hearts.
方法：采用高阻抗密封膜片箝全细胞技术，记录分离的豚鼠和家兔心肌单个细胞的钾电流。
Methods Enzyme digestion was performed to isolate single HERG-HEK cell and whole-cell patch-clamp technique was used to record HERG current and kinetic curves.
方法传代得到单个HERG-HEK细胞，应用全细胞膜片钳技术记录HERG-HEK细胞上的HERG钾电流和动力学曲线（激活、失活、复活和去活化）。
Methods Enzyme digestion was performed to isolate single HERG-HEK cell and whole-cell patch-clamp technique was used to record HERG current and kinetic curves.
膜片钳技术是一种以记录离子通道的离子电流来反映细胞膜上单一的或多个的离子通道分子活动的技术。
Methods Enzyme digestion was performed to isolate single ventricular myocyte of guinea pig and whole-cell patch-clamp technique was used to record potassium current.
方法酶解法分离豚鼠单个心室肌细胞，应用全细胞膜片钳技术研究白藜芦醇对豚鼠单个心室肌细胞钾电流的影响。
Methods Current-clamp recordings with the use of the whole cell configuration of the patch-clamp technique were made from the primary cultured spiral ganglion neurons.
方法应用膜片钳技术全细胞构型从原代培养的螺旋神经元获得电流钳记录。
As a result single guinea-pig atrial myocyte with normal electrophysiologic activity was obtained and its L-Ica and IK were successfully recorded with patch-clamp technique.
结果获得了具正常电生理活性的单个细胞及高阻封接的形成，成功记录了L -型钙流和延迟整流性钾流。
The effects of streptomycin on ionic currents in outer hair cells isolated from the cochlea of guinea pig were investigated using a whole-cell recording patch-clamp technique.
使用全细胞式记录膜片钳技术，研究链霉素对豚鼠耳蜗外毛细胞离子电流的影响。
Mechanical dissociation was used to obtain single primary spermatocytes from guinea pigs and iron channel currents in the primary spermatocytes were recorded by whole cell patch-clamp technique.
笔者应用机械分离获得豚鼠单个初级精母细胞，采用全细胞式膜片钳技术记录该细胞离子通道电流。
Using the whole-cell patch-clamp technique, clear evidence was provided for the existence of a hitherto unreported, voltage-dependent nonselective cation current (NSCC) in rabbit ventricular myocytes.
应用全细胞膜片钳制技术，我们首次报道了在兔的心室肌细胞存在一种电压依赖性的非特异性阳离子流（NSCC）。
METHODS Patch clamp technique with whole cell configuration.
方法膜片钳制技术全细胞记录模式。
METHODS The patch clamp whole cell recording technique was used.
方法采用膜片钳全细胞式记录技术。
The patch clamp technique bring about new light set in studying on mechanism of atherosclerotic development.
膜片钳技术给动脉粥样硬化发病机理研究带来了新的亮点。
The widely using of patch clamp technique has greatly promoted our knowledge of ion channels.
膜片钳技术的广泛应用极大推动了人们对离子通道的认识。
Patch clamp technique is a kind of technique which reflecting membrane molecular activity of one or more ion channel by record ion current of ion channel.
膜片钳技术是一种以记录离子通道的离子电流来反映细胞膜上单一的或多个的离子通道分子活动的技术。
Patch clamp technique and its application in Cardiovascular pharmacology, especially meticulous expounded ion channel about myocardium Were Summarized.
综述了膜片钳技术在心血管药理学中的应用，特别是对与心肌有关的离子通道做了细致地阐述。
Methods: Whole-cell patch clamp technique was used to determine sodium channel cur rent.
方法：采用全细胞膜片钳技术，记录钠通道电流。
The first step of recording calcium channel current of cardiomyocytes with patch clamp technique is dissociation of myocytes.
用膜片箝技术记录心肌细胞钙通道电流，首先必须获得单个心肌细胞。
Meynert nucleus was obtained and dissociated to observe their morphology and record electric activity by patch clamp technique.
作形态学观察，通过单细胞膜片钳技术记录电活动。
This article reviewed lately research status of Chinese herbal effect on calcium channels of myocardial cell by patch clamp technique and look ahead the future research.
本文综述了近年来利用膜片钳技术研究中药对心肌细胞膜钙通道作用的现状，并对未来研究进行展望。
Methods: The isolated neurons of dorsal root ganglion (DRG) were studied using the whole cell patch clamp technique in current clamp mode.
方法：应用全细胞膜片箝电流箝制技术，对急性分离的大鼠DRG细胞进行电流箝制。
CONCLUSION: the patch clamp technique makes it possible to observe the action sites of nicotine receptor antagonists.
结论：膜片钳技术使探讨烟碱受体拮抗药的作用位点成为可能。
Because the differences of its application, the microelectrode can be divided into ion-selective microelectrode and patch clamp detecting technique.
根据检测目的的不同，微电极主要应用可以分为离子选择微电极检测技术和膜片钳技术。
Methods The whole cell patch clamp technique was used in isolated ventricular cells of guinea pigs.
方法：用膜片钳实验技术记录胶原酶急性分离的豚鼠心室肌细胞钾通道电流。
The signal of single ion channel is stochastic ionic currents on the order of 1pA, which can be recorded by means of patch clamp technique.
细胞膜离子单通道信号是皮安级的随机电流，膜片钳技术可以记录这些信号。
Methods The data was recorded by adopting inside out configuration of patch clamp technique.
方法采用膜片钳技术内面向外式。
Single channel currents were recorded by using the patch clamp technique in inside-out configuration.
急性分离单个的豚鼠气管平滑肌细胞，以膜片钳技术的内面向外式记录单通道电流。
Methods Enzyme digestion was used to isolate single ventricular myocyte and whole cell patch clamp technique was used to record potassium current.
膜片钳技术是一种以记录离子通道的离子电流来反映细胞膜上单一的或多个的离子通道分子活动的技术。
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