Methods Enzyme digestion was performed to isolate single HERG-HEK cell and whole-cell patch-clamp technique was used to record HERG current and kinetic curves.
方法传代得到单个HERG-HEK细胞，应用全细胞膜片钳技术记录HERG-HEK细胞上的HERG钾电流和动力学曲线（激活、失活、复活和去活化）。
METHODS Patch clamp technique with whole cell configuration.
方法膜片钳制技术全细胞记录模式。
The patch clamp technique has become the main method for cell electrophysiology research.
膜片钳技术已成为细胞电生理学研究的重要手段。
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