Objective: To construct the prokaryotic expression vector of human heat shock protein 70(HSP 70) and to induce the expression and purification of HSP 70 in vitro.
目的：构建人热休克蛋白70（HSP 70）的原核表达载体，诱导其表达并纯化。
The part one of this thesis was concerned with expression and purification of recombinant low molecular weight sweet potato PAP and generation of a polyclonal antiserum from it.
本论文的第一部分致力于对低分子量的红薯紫色酸性磷酸酶的表达和纯化的研究，并产生相应的抗血清。
In this article, the latest bibliography were reviewed related to applications of detergents to in vitro expression, purification, and structural investigation of membrane proteins.
简要介绍了去垢剂在膜蛋白研究中的最新应用进展，步及去垢剂在膜蛋白离体表达、分离和纯化、以及结构研究中的应用。
Methods: Based on isolation, purification and expression of VEGF, successfully prepared anti VEGF antibody and developed a sandwich radioimmunoassay for VEGF.
方法：在表达及分离纯化VEGF的基础上，成功地制备了VEGF抗体，以夹心法测定vegf。
This thesis focus on the cloning, expression, purification and structural and functional studies of domains of human disease related proteins.
本论文工作的重点是与人类疾病相关的蛋白质的结构域的克隆表达及结构、功能的研究。
Cloning, expression, purification and functional study of human EGF in E. coli.
克隆、表达、纯化和人表皮生长因子在大肠杆菌中的功能研究。
Host Cell proteins are proteins and their modified forms derived from the hosts of biologics expression and co-exist during the biologics purification process.
宿主细胞蛋白质来源于生物纯化过程中宿主细胞产生的蛋白质及其蛋白质衍生物。
Affinity chromatography and ion exchange chromatography were applied in isolation and purification, and the bioactivity of expression protein was determined in cell proliferation test.
采用亲和层析和离子交换层析分离纯化，以细胞增殖实验测定表达蛋白的生物活性。
The study and application were introduced on the expression system in common use and separation and purification technique of recombinant proteins in recent years.
介绍了近年来重组蛋白质的常用表达系统以及各种分离纯化技术等研究和应用的进展情况。
Wang J. Li GC Expression, purification and identification of the human anti-idiotypic single chain antibodies against nasopharyngeal carcinoma in E. coli.
汪静。李官成。童永清鼻咽癌人源抗独特型基因工程抗体的原核表达及鉴定。
For the convenience of production and purification, Bacillus subtilis and Pichia pastor is were used as hosts for the expression of protease WF146.
为了获得大量有活性的重组蛋白酶便于今后的工业应用，我们将蛋白酶WF146克隆到分泌性表达宿主枯草芽胞杆菌和巴斯德毕赤酵母中。
For the convenience of production and purification, Bacillus subtilis and Pichia pastor is were used as hosts for the expression of protease WF146.
为了获得大量有活性的重组蛋白酶便于今后的工业应用，我们将蛋白酶WF146克隆到分泌性表达宿主枯草芽胞杆菌和巴斯德毕赤酵母中。
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