Objective: To construct the prokaryotic expression vector of human heat shock protein 70(HSP 70) and to induce the expression and purification of HSP 70 in vitro.
目的：构建人热休克蛋白70（HSP 70）的原核表达载体，诱导其表达并纯化。
The part one of this thesis was concerned with expression and purification of recombinant low molecular weight sweet potato PAP and generation of a polyclonal antiserum from it.
本论文的第一部分致力于对低分子量的红薯紫色酸性磷酸酶的表达和纯化的研究，并产生相应的抗血清。
Methods: Based on isolation, purification and expression of VEGF, successfully prepared anti VEGF antibody and developed a sandwich radioimmunoassay for VEGF.
方法：在表达及分离纯化VEGF的基础上，成功地制备了VEGF抗体，以夹心法测定vegf。
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