Method: RRTP-1 was obtained by ion exchange column chromatography and gel chromatography.
方法：采用离子交换柱层析和凝胶柱层析纯化得到RRTP；
For each pair of amino acids, determine which will be eluted first from the ion-exchange column using a pH 7.0 buffer.
确定下列每对氨基酸在pH7.0的缓冲液中，哪个先从离子交换柱上洗脱下来。
A weak anion exchange column was prepared and a separation method of interleukin-18 (IL-18) was developed on the column.
制备了弱阴离子交换整体柱，并建立了白介素18在该柱上分离纯化的方法。
The retention behaviour and mechanism of organic acids on an anion exchange column Shim pack IC A1 (Shimadzu, Japan) have been studied.
研究了有机酸在阴离子交换树脂上的保留行为。
Methods The peptide was separated by ion exchange column chromatography and further purified by reverse phase HPLC, and the molecular weight was determined by tricine gel electro phoresis.
用离子交换柱层析法分离，用反相高效液相色谱法进一步纯化，经聚丙烯酰胺凝胶电泳分析确定相对分子质量。
Methods The peptide was separated by ion exchange column chromatography and further purified by reverse phase HPLC, and the molecular weight was determined by tricine gel electro phoresis.
用离子交换柱层析法分离，用反相高效液相色谱法进一步纯化，经聚丙烯酰胺凝胶电泳分析确定相对分子质量。
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