Method: RRTP-1 was obtained by ion exchange column chromatography and gel chromatography.

方法：采用离子交换柱层析和凝胶柱层析纯化得到RRTP；

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For each pair of amino acids, determine which will be eluted first from the ion-exchange column using a pH 7.0 buffer.

确定下列每对氨基酸在pH7.0的缓冲液中，哪个先从离子交换柱上洗脱下来。

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A weak anion exchange column was prepared and a separation method of interleukin-18 (IL-18) was developed on the column.

制备了弱阴离子交换整体柱，并建立了白介素18在该柱上分离纯化的方法。

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