Add 950 ul lysis to the cell pellet and lyse again as step 1.
加入950ul溶菌buffer到细胞中如步骤一再裂解一次。
We first observed that the head ofmature tentacle contained transfer cells which were pellet shape, thickness cytoplast, large nucleolus, developing endoplasm, small vacuole and thick cell wall.
首次观察到成熟腺毛头部为具有独特的内突生长的传递细胞，细胞为圆球形，细胞质稠密，细胞核大，内质网发达，液泡较小，细胞壁厚。
Carefully transfer the supernatant of the flow-through to a new microcentrifuge tube (not supplied) without disturbing the cell-debris pellet in the collection tube.
小心将液体的上清移至一新的微量离心管中，不要搅动收集管中的细胞残渣沉淀。
Carefully transfer the supernatant of the flow-through to a new microcentrifuge tube (not supplied) without disturbing the cell-debris pellet in the collection tube.
小心将液体的上清移至一新的微量离心管中，不要搅动收集管中的细胞残渣沉淀。
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