Add 950 ul lysis to the cell pellet and lyse again as step 1.

加入950ul溶菌buffer到细胞中如步骤一再裂解一次。

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We first observed that the head ofmature tentacle contained transfer cells which were pellet shape, thickness cytoplast, large nucleolus, developing endoplasm, small vacuole and thick cell wall.

首次观察到成熟腺毛头部为具有独特的内突生长的传递细胞，细胞为圆球形，细胞质稠密，细胞核大，内质网发达，液泡较小，细胞壁厚。

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Carefully transfer the supernatant of the flow-through to a new microcentrifuge tube (not supplied) without disturbing the cell-debris pellet in the collection tube.

小心将液体的上清移至一新的微量离心管中，不要搅动收集管中的细胞残渣沉淀。

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