The recombinant Bacmid containing fused ORF220 and EGFP gene was confirmed by PCR.
通过PCR确认了含有融合的ORF220和EGFP基因的重组芽孢杆菌酰胺。
The recombinant Bacmid containing fused ORF220 and EGFP gene was confirmed by PCR and then transfected into Sf21 cells.
用PCR鉴定含有ORF220和EGFP基因的重组质粒，提取纯化重组质粒并转染昆虫细胞进行表达。
The recombinant Bacmid containing fused ORF220 and EGFP gene was confirmed by PCR and then transfected into Sf21 cells.
用PCR鉴定含有ORF220和EGFP基因的重组质粒，提取纯化重组质粒并转染昆虫细胞进行表达。
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