The recombinant Bacmid containing fused ORF220 and EGFP gene was confirmed by PCR.

通过 PCR 确认了含有融合的 ORF220 和 EGFP 基因的重组芽孢杆菌酰胺。

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The recombinant Bacmid containing fused ORF220 and EGFP gene was confirmed by PCR and then transfected into Sf21 cells.

用PCR鉴定含有ORF220和EGFP基因的重组质粒，提取纯化重组质粒并转染昆虫细胞进行表达。

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