You don't need to reply at once.
你不需要立即回复。
Please reply at your earliest convenience.
请尽早答复。
We shall be grateful if you will reply at an early date.
敬请早日答复，不胜感激。（望早日答复为盼。）
We would appreciate a reply at your earliest convenience.
我们希望您能尽快予以答复。
Kuei Chang-lin did not reply at once.
桂长林并不立刻回答。
We shall be grateful if you would reply at an early date.
敬请早日答复，将不胜感激。
We hope that you will give us your reply at an early date.
我方希望贵方能早日答复我方。
I shall feel obliged by a reply at your earliest convenience.
如能尽早收到你的回复我将非常感谢。
Please reply at once in order not to lose this opportunity of a lower price.
请立即回复，以免失去这次低价的机会。
We expected that you will give us a definite reply at your earliest convenience.
期盼贵方能尽早给我方一个明确的答复。
We expected that you will give us a definite reply at your earliest convenience.
期盼贵方能尽早给我方一个明确的答复。
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