You would first get exposed in children-- in childhood and then at a point when you're susceptible to the disease.

在儿童期这个较容易受病毒感染时期,第一次接触该病毒,就会引起发病

耶鲁公开课 - 生物医学工程探索课程节选

Right, but if you just got the primary exposure, wouldn't the secondary response automatically-- not a booster shot, just the secondary response, because you were exposed?

但这时你已经获得这样的初次免疫,而因为你已经天然感染过该病毒,那么,在没有加强针的情况下,二次免疫是否无法自动发生

耶鲁公开课 - 生物医学工程探索课程节选

Well, what if you could hijack that virus to carry genes that you want?

那么,如果你能,挟持病毒使之携带你所要的基因该多好

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Took it out of the virus completely, cloned it into a plasmid, that plasmid was expressed in a foreign host, in this case it was expressed in yeast cells.

将基因从病毒中完全提取出来,并克隆制成质粒,该质粒在外来宿主中表达,此例中质粒在酵母细胞中表达

耶鲁公开课 - 生物医学工程探索课程节选

Hopefully their immune systems will respond to it like the real virus but it won't be capable of replication because we've chemically cross linked it so it can't go through its life cycle.

人体的免疫系统,就会对该病毒产生免疫应答,但灭活的病毒不能复制,因为我们已经对它做了化学处理,使它无法进入生命周期

耶鲁公开课 - 生物医学工程探索课程节选