Or you could ask the question, I have this restriction enzyme, at what regions on this plasmid will it cut?

你也可以这样问,我的这种限制性内切酶,会从质粒的哪个区域切开呢

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If we injected it into a muscle let's say, and your muscle cells took up this plasmid.

比如说,如果我们将其注射入肌肉内,则肌肉细胞就可以吸收这些质粒

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If I cut both the plasmid and my DNA of interest with the same restriction enzyme I'm going to end up with the same sticky ends on both molecules.

如果用同一种限制性内切酶,来切割质粒和我感兴趣的DNA,在两个分子上就能得到同样的粘性末端

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Well the first step would be to cut open the plasmid with a particular restriction enzyme, and then what if I take that same restriction enzyme and I cut up the DNA that I'm interested in.

第一步是用某种限制性内切酶把质粒切开,然后用同一种限制性内切酶,切出我想要的DNA

耶鲁公开课 - 生物医学工程探索课程节选

Now if I put them in contact with one another, the plasmid that's been opened and fragments of the DNA - special fragments that I've produced with the same restriction enzyme, they'll have the same sticky ends, they will naturally hybridize with one another.

如果我让它们互相接触,已经切开的质粒和DNA片段--,这DNA片段是我已用同种限制性内切酶,处理过的特别片段,这两者会有相同的粘性末端,它们会自然地相互杂交

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