The first step in the process is to take our plasmid which we've selected, and to insert the gene that we want into it.

这个过程的第一步,是取得我们选择的质粒,把我们需要的基因插入到里面

耶鲁公开课 - 生物医学工程探索课程节选

The first step is we had to be able to take this circular DNA and cut it to create a site for our new gene to be added.

第一步是切割环形DNA,以创造出一个整合新基因的位点

耶鲁公开课 - 生物医学工程探索课程节选

So in the first week we run a section called from strawberries to gene therapy where we talk about DNA, extract DNA, you can play with the DNA of an organism and we can think about how to use DNA for other purposes.

第一周组会的主题是从草莓到基因疗法,主要讨论DNA和DNA的提取,使你们对有机组织的DNA有直观的认识,并思考DNA的其它应用

耶鲁公开课 - 生物医学工程探索课程节选