Or you could ask the question, I have this restriction enzyme, at what regions on this plasmid will it cut?

你也可以这样问,我的这种限制性内切酶,会从质粒的哪个区域切开呢

耶鲁公开课 - 生物医学工程探索课程节选

And just to give you a sense, if you do a cross-cut of this, you cannot show it from this angle, but it should really be sort of a dumbbell shape with the nodal plane in between the two nuclei.

告诉你们这些就是给你们点概念，假如你们把他横切切开，不能从角度上展示，但它还是会保留几分哑铃的形态,以及在两核之间的节面。

麻省理工公开课 - 固态化学导论课程节选

Take a knife and open up the skin and let's see what's inside.

用刀子切开皮肤,看看体内有什么

耶鲁公开课 - 生物医学工程探索课程节选

Here is a cartoon which actually illustrates a classic experiment by some physiologists who for some reason had a dog's brain opened up and started shocking different parts of the brain.

这是张卡通图画,实际上描绘了一个由生理学家,所做过的经典实验,出于某种原因,他们切开一只狗的大脑,对不同的大脑区域进行电击

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So if you have plasmid where you know all the base pairings than you could go through that plasmid and say I want to cut it right here.

如果你已经知道某个质粒上的所有序列,你就可以决定从哪里切开质粒

耶鲁公开课 - 生物医学工程探索课程节选

Take a plasmid, cut it open, insert a gene that we want into the plasmid, and then put that plasmid in a host cell, and let the host replicate it.

取一个质粒,把它切开,把目标基因放进去,然后把这个质粒植入到宿主细胞,让宿主来进行复制

耶鲁公开课 - 生物医学工程探索课程节选

That is,it cuts between the G and the A here, and it cuts between the G and the A here.

就是说,它从这里的G和A之间切开,从这里的G和A之间切开

耶鲁公开课 - 生物医学工程探索课程节选

Well the first step would be to cut open the plasmid with a particular restriction enzyme, and then what if I take that same restriction enzyme and I cut up the DNA that I'm interested in.

第一步是用某种限制性内切酶把质粒切开,然后用同一种限制性内切酶,切出我想要的DNA

耶鲁公开课 - 生物医学工程探索课程节选

The first step is to take the DNA fragments that we're interested in and put them into this vector by basically cutting open the double stranded DNA and inserting the gene that we like in the region where we've cut.

第一步是把我们感兴趣的DNA片段,整合到这个载体里,基本的方法是切开质粒这个双链DNA,把我们想要的基因插入切开的区域

耶鲁公开课 - 生物医学工程探索课程节选

Now if I put them in contact with one another, the plasmid that's been opened and fragments of the DNA - special fragments that I've produced with the same restriction enzyme, they'll have the same sticky ends, they will naturally hybridize with one another.

如果我让它们互相接触,已经切开的质粒和DNA片段--,这DNA片段是我已用同种限制性内切酶,处理过的特别片段,这两者会有相同的粘性末端,它们会自然地相互杂交

耶鲁公开课 - 生物医学工程探索课程节选