The second issue is that the gene vector, in this case the plasmid, has to be compatible with the cells that you're trying to express the gene in.

接下来的问题是如何选择合适的基因载体,在这里用到的,质粒,必须与要用来表达基因的,受体细胞匹配

耶鲁公开课 - 生物医学工程探索课程节选

Then we're going to take these plasmid vectors and we're going to somehow put them in contact with cells in such a way that the cells ingest the DNA and they use it.

然后要使这些质粒载体,以一种特别的方式与细胞接触,让细胞吞入质粒载体并使之表达

耶鲁公开课 - 生物医学工程探索课程节选

Eventually what happens is that glucose transport molecules which are expressed and stored inside the cell get shuttled up to the surface, so the cells permeability to glucose goes up and more glucose can come in.

最终的结果是那些已经表达,并储存于细胞中的能够运输糖的载体分子,穿梭到细胞表面,这样细胞对糖的透过性提高,更多糖分子进入到细胞内部

耶鲁公开课 - 生物医学工程探索课程节选