Get a gene, put it in a plasmid, get that plasmid expressed in a host, could even be a bacterial host,right?

把一个基因导入一个质粒,让质粒在宿主中表达,这个宿主甚至可以是细菌,对吧

耶鲁公开课 - 生物医学工程探索课程节选

It's our host, it's our mother,

她毕竟是我们的主人，我们的母亲，

有很多活动 - SpeakingMax英语口语达人

The only way that we knew how to grow it reliably was to grow it in its natural host: cows.

那目前我们所知道的唯一途径就是,利用它的自然宿主,奶牛

耶鲁公开课 - 生物医学工程探索课程节选

It seemed I had a whole host of memories going back to 1750 in England and that I was in San Francisco now only in another life and in another body.

似乎全部记忆都回到了1750年的英格兰,而现在在圣弗兰西斯科的我则是另一个人，在另一种生活里。

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By extending your time horizon to three years, or four years,or five years, it opens up a whole host of investment opportunities that aren't available to people that are playing this silly,short-term game.

只有通过将投资延长至三年,四年乃至五年,才会出现大量的获利机会,沉迷于愚蠢的短线操作,是无法得到这些机会的

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Took it out of the virus completely, cloned it into a plasmid, that plasmid was expressed in a foreign host, in this case it was expressed in yeast cells.

将基因从病毒中完全提取出来,并克隆制成质粒,该质粒在外来宿主中表达,此例中质粒在酵母细胞中表达

耶鲁公开课 - 生物医学工程探索课程节选

Otherwise you could have a squirrel host-- I'm using squirrels, because I think that's funnier than other animals to think of— but it could be any potential host, right?

否则松鼠就会变成宿主,我拿松鼠举例,是因为我觉得它比别的动物更有趣,但潜在宿主可以是任何动物,对吧

耶鲁公开课 - 生物医学工程探索课程节选

Take a plasmid, cut it open, insert a gene that we want into the plasmid, and then put that plasmid in a host cell, and let the host replicate it.

取一个质粒,把它切开,把目标基因放进去,然后把这个质粒植入到宿主细胞,让宿主来进行复制

耶鲁公开课 - 生物医学工程探索课程节选

It's foreign, it's not part of the host or not a normal part of the host.

所以它成了外源物,外源物不是宿主的一部分,至少不是正常的一部分

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Now in this case the host - you want the host not only to replicate all the DNA, you want it also to express the gene.

而这种情况下宿主--,你不只希望宿主复制基因,同时也想使之表达基因

耶鲁公开课 - 生物医学工程探索课程节选

Some words that are useful in this discussion, a 'host'. A host is the organism that you're interested in defending and it could be you or me or some typical person.

讨论中会用到一些词,宿主 宿主是,你对其免疫反应感兴趣的生物体,可以是你我,或者其他人

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So, when the right signal is received, the right T-cell finds your host cell with a foreign virus in it, the first thing that happens is that this T-cell becomes activated and it starts reproducing, making more copies of itself.

所以 当接收到正确的信号,恰当的T细胞寻觅到,感染外源病毒的细胞时,首先发生的是,T细胞被激活并开始增殖,制造更多自身拷贝

耶鲁公开课 - 生物医学工程探索课程节选