Get a gene, put it in a plasmid, get that plasmid expressed in a host, could even be a bacterial host,right?

把一个基因导入一个质粒,让质粒在宿主中表达,这个宿主甚至可以是细菌,对吧

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Now in this case the host - you want the host not only to replicate all the DNA, you want it also to express the gene.

而这种情况下宿主--,你不只希望宿主复制基因,同时也想使之表达基因

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Take a plasmid, cut it open, insert a gene that we want into the plasmid, and then put that plasmid in a host cell, and let the host replicate it.

取一个质粒,把它切开,把目标基因放进去,然后把这个质粒植入到宿主细胞,让宿主来进行复制

耶鲁公开课 - 生物医学工程探索课程节选