Until now,little has been known about the transcription of intronic miRNA and its relationship with host gene.
目前,对miRNA基因转录的了解仍十分有限,而对位内含子区域的miRNA及其与宿主基因的转录关系,更是知之甚少。
参考来源 - 位于可变内含子区的果蝇microRNA·2,447,543篇论文数据,部分数据来源于NoteExpress
Phylogenetic analysis of IAP genes and lots of evidence sppport the origin of this viral gene by capture of a host gene early in the evolution of Lepidoptera.
系统分析表明,杆状病毒IAP基因可能是病毒与鳞翅目昆虫在长期的进化过程中从宿主基因组中获得的。
Get a gene, put it in a plasmid, get that plasmid expressed in a host, could even be a bacterial host,right?
把一个基因导入一个质粒,让质粒在宿主中表达,这个宿主甚至可以是细菌,对吧
Now in this case the host - you want the host not only to replicate all the DNA, you want it also to express the gene.
而这种情况下宿主--,你不只希望宿主复制基因,同时也想使之表达基因
Take a plasmid, cut it open, insert a gene that we want into the plasmid, and then put that plasmid in a host cell, and let the host replicate it.
取一个质粒,把它切开,把目标基因放进去,然后把这个质粒植入到宿主细胞,让宿主来进行复制
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