Magnetic absorption cell sorting (MACS) method was used to isolate the CD4 + t lymphocytes from the spleen of the rats. Flow cytometry was used to monitor the efficiency of isolation.
利用磁吸附细胞分选法(MACS)分离大鼠脾脏CD 4 +T细胞，流式细胞术检测MACS分选前后CD 4 + T细胞比例，监测细胞分选效果。
Method: Apoptosis was detected by the morphologic observation, flow cytometry assay and DNA gel electrophoresis.
方法：应用细胞形态学、流式细胞仪分析及DNA凝胶电泳等。
Conclusions The reticulocyte counting by flow cytometry is a rapid, objective, accurate and precise method and is appropriate for routine clinical detection.
结论流式细胞仪计数网织红细胞快速、客观、结果准确、重复性好，适合临床常规检测网织红细胞。
Conclusion The reticulocyte counting by flow cytometry is a rapid, accurate and precise method and is appropriate for routine clinical detection.
结论流式细胞仪计数网织红细胞快速，结果准确，且重复性好，适合临床常规检测网织红细胞。
Objective To evaluate the method of determining the proliferation of human T lymphocyte subpopulations by using flow cytometry and carboxyfluorescein diacetate succinimidyl ester(CFSE) labeling assay.
目的探讨应用流式细胞术和活细胞荧光染料CFSE检测T淋巴细胞各亚群增殖反应的方法学。
Method Flow cytometry was used.
方法应用流式细胞仪检测。
The cell viability was determined by try pan blue exclusion assay and cell cycle analyzed by flow cytometry, with the ce ll apoptosis assayed by TUNEL method.
应用锥虫蓝染色测定细胞活力、流式细胞计数分析细胞周期、TUNEL分析细胞凋亡。
Conclusion Flow cytometry determining reticulocyte percentage and reticulocyte maturity index seems to be rapid, objective and precise method, and appropriate for clinical detection.
结论利用流式细胞仪进行网织红细胞百分率及网织红细胞成熟指数的检测，快速、客观、准确，可作为临床检验指标之一。
Results The C6 glioma cell apoptosis induction of GFP contained serum in vitro could be confirmed by morphological examination, flow cytometry analysis and TUNEL method.
结果形态学变化、流式细胞仪分析和TUNEL法结果均证实含鲜壁虎血清在体外可诱导C6胶质瘤细胞凋亡；
Methods The survival rate and apoptosis rate of QBC939 after being treated with different density of 5-aza-2'-deoxycytidine were detected by using the method of MTT and flow cytometry.
方法应用MTT法检测不同浓度的5-氮-2’-脱氧胞苷对胆管癌细胞株QBC939存活率的影响，应用流式细胞术检测5-氮-2’-脱氧胞苷对QBC939细胞生长周期及凋亡率的影响。
Objective to establish the method of the reticulocyte counting by flow cytometry and to apply in clinical laboratory.
目的建立流式细胞仪计数网织红细胞的方法并应用于临床。
Method: Fetal rat microglia in brain was isolated and purified by lidocaine hydrochloride with shock culturing. The purification of microglia was detected by immunofluorescence and flow cytometry.
方法利用盐酸利多卡因注射液联合震荡培养法分离纯化胎大鼠脑内小胶质细胞，免疫荧光技术及流式细胞技术鉴定小胶质细胞的纯度。
Objective to establish a method for detection of circulating monocyte-platelet aggregates by flow cytometry.
目的建立流式细胞术检测循环全血中单核细胞与血小板聚合率的方法。
AIM: to set up an accurate and rapid method to detect the phenotype of peripheral blood eosinophils using flow cytometry.
目的：应用流式细胞术，建立一种准确、外周血嗜酸性细胞及其相关分子的快速测定方法。
Method: Using fluorescence microscopy, electron microscopy, laser scanning confocal microscopy, flow cytometry and DNA gel electrophoresis to demonstrate the presence of apoptosis.
方法：采用荧光显微镜、电子显微镜、激光扫描共聚焦显微镜、流式细胞分光光度计和DNA凝胶电泳等方法和技术观察细胞凋亡。
The invention discloses a flow cytometry and a waste liquid eductor thereof and a method.
本发明公开了一种流式细胞仪及其废液排放装置和方法。
Proliferating cell nuclear antigen (PCNA) expression of colon tissue was determined by immunohistochemical method, the changes of cell cycle was tested by flow cytometry.
疗程结束后，取大鼠结肠组织，免疫组化方法检测其增殖细胞核抗原(pcna)的表达，流式细胞仪分析其细胞周期的变化。
The fluorescence flow cytometry (FCM) DNA assay and TUNEL were applied to detect cell cycle phase and cell apoptosis. the endogenous VEGF contents were detected by ELISA method.
应用流式细胞术进行DNA倍体分析和TUNEL分析，检测两药单用与联用对细胞周期及早期凋亡的影响；
The fluorescence flow cytometry (FCM) DNA assay and TUNEL were applied to detect cell cycle phase and cell apoptosis. the endogenous VEGF contents were detected by ELISA method.
应用流式细胞术进行DNA倍体分析和TUNEL分析，检测两药单用与联用对细胞周期及早期凋亡的影响；
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