Magnetic absorption cell sorting (MACS) method was used to isolate the CD4 + t lymphocytes from the spleen of the rats. Flow cytometry was used to monitor the efficiency of isolation.
利用磁吸附细胞分选法(MACS)分离大鼠脾脏CD 4 + T细胞，流式细胞术检测MACS分选前后CD 4 + T细胞比例，监测细胞分选效果。
Method: Apoptosis was detected by the morphologic observation, flow cytometry assay and DNA gel electrophoresis.
方法：应用细胞形态学、流式细胞仪分析及DNA凝胶电泳等。
Conclusions The reticulocyte counting by flow cytometry is a rapid, objective, accurate and precise method and is appropriate for routine clinical detection.
结论流式细胞仪计数网织红细胞快速、客观、结果准确、重复性好，适合临床常规检测网织红细胞。
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