Aim: To develop a PCR technique for rapid screening of recombinant plasmid in subtractive library of cDNA.
目的：消减文库构建过程中，用P CR技术快速筛选重组阳性克隆。
Conclusion: The DNA subtractive library may provide a solid foundation for screening and cloning pathogenic genes of UPEC132.
结论：所构建的基因组d NA消减文库为进一步筛选UPEC132中与致病相关基因奠定了基础。
Recombinant clone can be analyzed directly with the use of PCR. It is very efficient, especially in establishment of subtractive library of cDNA.
筛选重组阳性克隆可直接用细菌悬液作PCR模板，在消减文库构建时，能大大提高工作效率。
In order to study the leaf-specific expressed genes in Oryza sativa L., the subtracted library of rice leaf was established by suppression subtractive hybridization (SSH).
为了研究水稻叶组织特异基因及其表达，利用抑制消减杂交法(SSH)建立了水稻叶对根的差减文库。
In order to study the leaf-specific expressed genes in Oryza sativa L., the subtracted library of rice leaf was established by suppression subtractive hybridization (SSH).
为了研究水稻叶组织特异基因及其表达，利用抑制消减杂交法(SSH)建立了水稻叶对根的差减文库。
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