Aim: To develop a PCR technique for rapid screening of recombinant plasmid in subtractive library of cDNA.
目的：消减文库构建过程中，用P CR技术快速筛选重组阳性克隆。
Conclusion: The DNA subtractive library may provide a solid foundation for screening and cloning pathogenic genes of UPEC132.
结论：所构建的基因组d NA消减文库为进一步筛选UPEC132中与致病相关基因奠定了基础。
Recombinant clone can be analyzed directly with the use of PCR. It is very efficient, especially in establishment of subtractive library of cDNA.
筛选重组阳性克隆可直接用细菌悬液作PCR模板，在消减文库构建时，能大大提高工作效率。
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