This research intended to construct a eukaryotic expression vector with a site-directed mutation of porcine MSTN propeptide gene.
本研究旨在构建具有猪 MSTN 前肽基因定点突变的真核表达载体。
Site-directed mutagenesis is one of the key methods used to study gene function.
定点突变是研究基因功能的一种关键方法。
Objective: To develop a simple and efficient way to perform site-directed mutagenesis.
目的：介绍一种简便、有效的定点突变技术。
In the past, the Affinity Labeling and Site-Directed Mutagenesis are the primary methods to study amine receptor.
过去主要是通过亲和标记和点突变等实验方法进行研究。
The results indicated that some site-directed mutagenesis can reveal important functional domains of enzyme activity.
结果表明某些定点突变可以揭示影响酶活的重要功能域。
To clarify the regulatory mechanism, site-directed mutagenesis and reporter gene assay in the CHO cell line were used.
为了阐明调控机制，我们采用了点突变实验和在CHO细胞系中的报告基因分析。
Methods The BO mutants I402G and C457S were obtained by site-directed mutagenesis and confirmed by amino acid sequence analysis.
方法胆红素氧化酶突变体i 402g和C457S通过聚合酶链反应获得，并经氨基酸序列测定加以证实。
The principles, operation and applications of site-directed mutagenesis, gene fusion technology, and post-translational modification methods were introduced emphatically.
着重阐述了基因定点突变技术、基因融合技术和翻译修饰技术等新兴定点固定化技术的原理、特点和操作。
The reliability of the method was confirmed by site-directed mutagenesis, selective chemical modification, site-specific antipeptide antibody binding experiments, and so on.
大量定点突变实验、选择性化学修饰实验、抗多肽抗体结合实验等能很好地确证该联配结果的正确性。
Longer distance constraints are determined by site-directed spin labelling electron paramagnetic resonance, but current labelling strategies still constitute some limitation.
直接位点标记的旋转电子顺磁共振可以得到更长的距离约束数据，但是目前的标记策略仍然具有局限性。
This research intended to construct eukaryotic expression vector with a site-directed mutation of porcine MSTN propeptide gene, and verify its expression efficacy in C2C12 cells.
本研究旨在克隆通城猪含有第1个内含子的MSTN前肽基因，构建真核定点诱变载体，并通过转染C2C12细胞验证载体表达的有效性。
Methods The polyadenylation signal-deficient retrovirus vector mutated by PCR site-directed mutagenesis was used to make polyadenylation signal-deficient retroviruses by PA317 packaging cells.
方法使用人工定点突变多聚腺苷酸化信号的小鼠逆转录病毒载体，应用PA317病毒包装细胞获得多聚腺苷酸信号缺陷的重组逆转录病毒；
Site-directed mutagenesis and single-channel patch-clamp recording were used to explore the molecular transitions that underlie inactivation in Shaker potassium channels expressed in Xenopus oocytes.
定点基因突变以及单通道夹被用于探测爪蟾卵细胞中摇动钾离子通道被去活化时的分子转移过程。
And then, you can make sure everything on your site and in your app is directed at that core function.
然后，确保您的站点上和应用程序中的所有东西都以该核心功能为中心。
Thus, a user might click on a "secure" URL on your page and actually be directed to an intruder's site.
因此，用户可能会单击页面上看似安全的URL，但是他实际上会被转到入侵者的站点上。
When she returned a lost cell phone to an oddly dressed salesman, she mentioned wanting a boyfriend. To thank her, he directed her to his company's website, which is a site to create your own lover.
当她将丢失的手机还给一个穿着古怪的推销员时，她提到想拥有一个男朋友。
What happens in a multiserver site if a client accesses a first server (and establishes a session with it) and then is directed (by a load balancer) to a second server?
如果一个客户访问第一个服务器(并建立一个会话跟它一起)，然后被(负载均衡服务器)导向到第二个服务器，那么在一个多服务器站点将会发生什么？
After completing a product search, users are directed to a co-branded site within Yahoo Travel to continue with further searches, product filters and bookings.
在完成了产品搜索之后，用户将被引导到Yahoo旅游频道内部一个有双方品牌的页面来进行更加深入的搜索、产品过滤和预订。
Because the gently sloped site is mostly surrounded by dense foliage, all of the momentum of the house is directed toward the sky.
由于轻轻倾斜网站主要是围绕着茂密的森林，对房子的势头一切是为了天空。
The scheme is accessible for the surrounding neighborhood, with sightlines from adjacent streets directed towards the heart of the site.
周围的街区可访问该基地，邻近街道的视线正对场地的中心。
AIM: To establish the mutant of coding calcium binding fragment of the 13th exon of human thrombospondin-1 (TSP-1) gene with polymerase chain reaction (PCR) site directed mutagenesis technology.
目的：利用聚合酶链反应定点突变技术构建人血小板反应素1基因第13外显子编码钙结合域突变体。
I had traveled here to Henson's last home — now a historic site that Carter formerly directed — to learn more about a man who was, in many ways, an African-American Moses.
我远道前来亨森最后的居所——如今已成为卡特曾管理过的一处历史遗迹——是为了更多地了解此人，他在许多方面堪称黑人摩西。
Create a custom 404 page so that even if someone encounters an error on your site, they are re-directed somewhere nice.
创建404错误页面，一旦你的网站出现问题，给别人一个好的体验。
The Site is not directed to any person in any jurisdiction where the publication or availability of the Site is prohibited, by reason of that person's nationality, residence or otherwise.
网站不是针对禁止网站的发布或接达的任何司法管辖区的任何人，而不论其禁止是因为该人的国籍、居住资格，或是因为其它原因。
The mutant G138P was obtained by in vitro site directed mutagenesis of GI gene.
用双引物法对GI基因进行体外定点突变，构建了GI突变体g 138p。
To thank her, he directed her to his company's website, which is a site to create your own lover. Thinking it is a game, she ordered one.
作为答谢，这个推销员指引梨衣子登录了他公司的网站，在这里可以创造自己的专属情人。
In 1958, he has directed the "youth site" and three films.
1958年，他先后导演了《工地青年》等三部影片。
The illustrative embodiments described herein are directed to an apparatus and method for managing reduced pressure at a tissue site.
本文描述的说明性实施方式涉及用于管理组织部位处的减压的装置和方法。
The illustrative embodiments described herein are directed to an apparatus and method for managing reduced pressure at a tissue site.
本文描述的说明性实施方式涉及用于管理组织部位处的减压的装置和方法。
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