Construction of L60V and I97L mutants and recombinant adenovirusesBy site-directed mutagenesis technique, p3.8llplasmid, which contains 1.2 copies of HBV genome, was used as template to produce the two desired mutations.
1．重组腺病毒的构建 用定点诱变技术，以含有1.2拷贝HBV基因组的p3.8Ⅱ质粒为模板，构建含L60V和I97L的突变株。
参考来源 - 用腺病毒载体介导的基因转移研究HBV/C区热点变异对小鼠免疫应答的影响·2,447,543篇论文数据，部分数据来源于NoteExpress
This research intended to construct a eukaryotic expression vector with a site-directed mutation of porcine MSTN propeptide gene.
本研究旨在构建具有猪 MSTN 前肽基因定点突变的真核表达载体。
Site-directed mutagenesis is one of the key methods used to study gene function.
定点突变是研究基因功能的一种关键方法。
Objective: To develop a simple and efficient way to perform site-directed mutagenesis.
目的：介绍一种简便、有效的定点突变技术。
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