Methods: the blood samples were collected from HFRS patients between 2000 ~ 2001, and the m segment genome of hantavirus was amplified by RT-PCR.
方法:采集2000 ~ 2001年肾综合征出血热(HFRS)患者的血液标本,通过逆转录聚合酶链反应(RT - PCR)扩增汉坦病毒M片断基因。
Methods The L and M segment cDNA of Hantavirus Q32 strain was amplified by RT-PCR. The purified PCR products were sequenced directly or cloned into pGEM-T Vector and then sequenced.
方法设计特异的PCR引物,用RT-PCR技术分段扩增Q32株全长L、M片段,PCR产物纯化后直接测序,或用T-A克隆方法进行PCR产物克隆,然后进行遗传进化分析。
As a special case of the previous example, a segment of integers, [n.. m] can be thought of as a category.
上边例子的特殊情况一个整数片断[n. .M]可以被认为是一个范畴。
One high m. p. g. segment where Detroit still lags is alternative fuel vehicles.
底特律尚落后的,是在高燃油经济的替代燃料汽车这一细分市场。
Compared with traditional optical flow and m estimation methods, the proposed algorithm only calculates in the DC domain and does not iterate, so it can segment almost in real time.
与传统光流法、M估计法比较,该算法只在压缩域上进行,不需迭代,具较强实时性。
The north segment of the west branch fault and the southeast segment of the east branch fault in the Xiaojing fault zone possess high potential magnitudes close to M 7.
潜在地震震级较高的段有小江西支断裂北段和小江东支断裂东南段, 其潜在震级都接近7 级。
The north segment of the west branch fault and the southeast segment of the east branch fault in the Xiaojing fault zone possess high potential magnitudes close to M 7.
潜在地震震级较高的段有小江西支断裂北段和小江东支断裂东南段, 其潜在震级都接近7 级。
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