Methods: the blood samples were collected from HFRS patients between 2000 ~ 2001, and the m segment genome of hantavirus was amplified by RT-PCR.
方法:采集2000 ~ 2001年肾综合征出血热(HFRS)患者的血液标本,通过逆转录聚合酶链反应(RT - PCR)扩增汉坦病毒M片断基因。
Methods The L and M segment cDNA of Hantavirus Q32 strain was amplified by RT-PCR. The purified PCR products were sequenced directly or cloned into pGEM-T Vector and then sequenced.
方法设计特异的PCR引物,用RT-PCR技术分段扩增Q32株全长L、M片段,PCR产物纯化后直接测序,或用T-A克隆方法进行PCR产物克隆,然后进行遗传进化分析。
One high m. p. g. segment where Detroit still lags is alternative fuel vehicles.
底特律尚落后的,是在高燃油经济的替代燃料汽车这一细分市场。
I'm going to build, this case, a Cartesian point, I'm going to build a second Cartesian point, and my segment passes in those class instances.
我要去建立,在这个例子中,建立一个笛卡尔点,然后再建立一个笛卡尔点,然后向我的segment类。
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