Rat tracheal epithelial cell (RTE) grows well in vitro under the support of 3t3 feeder cell layer.
原代rte细胞在3T3滋养细胞支持下，在体外培养条件下可生长。
The effect of feeder cell type, growth factors, and embryo developmental stage on establishment of sheep embryonic stem cell was also studied in present experiment.
同时，比较了饲养层细胞类型、生长因子、胚胎发育阶段等因素对绵羊胚胎干细胞分离培养效果的影响。
Objective: Sepretae Sertoli cell, BMSC, MEF, Pancreatic fibroblast and establish cell lines, constructed different feeder layers for a source as a coculture system for stem cells.
目的： 分离培养睾丸支持细胞、骨髓基质细胞、胚胎成纤维细胞和胰腺成纤维细胞，优化培养条件，建立饲养细胞系，为干细胞体外共培养体系的建立提空充足的饲养细胞。
Objective to explore the optimal condition of direct differentiation into dopaminergic neurons of embryonic stem cells in serum-free and feeder layer cell free medium.
目的寻求无血清、无饲养层细胞存在的情况下，胚胎干细胞向多巴胺能神经元定向诱导分化的最佳条件。
Selection of the feeder layer, medium, and supplemental cytokines and their roles in establishing and maintaining human ES and EG cell lines at present are illustrated in detail systematically.
详细系统地说明了迄今为止建立人胚胎干细胞系所需要的饲养层类型、培养基组成、添加细胞因子种类及其作用；
Without feeder layer, human embryonic stem cell differentiation was observed in vitro.
撤除饲养层，观察人胚胎干细胞体外分化情况。
Without feeder layer, human embryonic stem cell differentiation was observed in vitro.
撤除饲养层，观察人胚胎干细胞体外分化情况。
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