Rat tracheal epithelial cell (RTE) grows well in vitro under the support of 3t3 feeder cell layer.
原代rte细胞在3 T3滋养细胞支持下,在体外培养条件下可生长。
The effect of feeder cell type, growth factors, and embryo developmental stage on establishment of sheep embryonic stem cell was also studied in present experiment.
同时,比较了饲养层细胞类型、生长因子、胚胎发育阶段等因素对绵羊胚胎干细胞分离培养效果的影响。
Objective: Sepretae Sertoli cell, BMSC, MEF, Pancreatic fibroblast and establish cell lines, constructed different feeder layers for a source as a coculture system for stem cells.
目的: 分离培养睾丸支持细胞、骨髓基质细胞、胚胎成纤维细胞和胰腺成纤维细胞,优化培养条件,建立饲养细胞系,为干细胞体外共培养体系的建立提空充足的饲养细胞。
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