Objective to study the influence of some factors on DNA cycle sequencing.
目的研究DNA循环测序中一些常见因素的影响。
Methods The effects of DNA templates, primers, cycle sequencing reactions as well as purification methods were comparatively analyzed.
方法对测序中模板、引物、测序反应条件及其产物的纯化等因素进行比较研究。
The effects of DNA templates, primers, cycle sequencing reaction conditions, purification methods, operation of instrument were comparatively analyzed.
对测序中的模板、引物、测序反应条件及测序反应纯化方法和仪器操作等进行研究。
Methods: PCRbased cycle DNA sequencing is a method that combines PCR amplification with DNA sequencing.
方法：PCR循环测序法是将PCR扩增与核酸序列分析相结合的一种研究方法。
Methods: PCRbased cycle DNA sequencing is a method that combines PCR amplification with DNA sequencing.
方法：PCR循环测序法是将PCR扩增与核酸序列分析相结合的一种研究方法。
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