PCR产物测序常采用循环测序法（cycle sequencing）：在PCR反应体系中同时将ddNTP加入，并利用同位素或荧光素标记的引物引导扩增，使模板的扩增与测序同时进行。
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thermal cycle sequencing 热循环测序
dsdna cycle sequencing 双链dna循环测序
dsDNA cycle sequencing system 双链DNA循环测序体系
dye terminator cycle sequencing 荧光终止法PCR循环测序
Objective to study the influence of some factors on DNA cycle sequencing.
目的研究DNA循环测序中一些常见因素的影响。
Methods The effects of DNA templates, primers, cycle sequencing reactions as well as purification methods were comparatively analyzed.
方法对测序中模板、引物、测序反应条件及其产物的纯化等因素进行比较研究。
The effects of DNA templates, primers, cycle sequencing reaction conditions, purification methods, operation of instrument were comparatively analyzed.
对测序中的模板、引物、测序反应条件及测序反应纯化方法和仪器操作等进行研究。
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