Objective: To study the expression of murine stem cell factor in CHO cells.
目的：克隆造血干细胞因子并在CHO细胞中表达。
To clarify the regulatory mechanism, site-directed mutagenesis and reporter gene assay in the CHO cell line were used.
为了阐明调控机制，我们采用了点突变实验和在CHO细胞系中的报告基因分析。
In comparison with commercial SFM, it can maintain growth of CHO cell for much longer peroid and the expressing level of HBsAg is higher too.
该培养基与商品化的无血清培养基比较，能够使细胞生长维持较长的时间，表达产物分泌量也相对较高。
The positive clones were selected by G418, and highly expressing clones were more selected by flow cytometry, and get stable highly expressing CHO cell lines.
用新霉素类似物(G418)筛选出阳性克隆，并用流式细胞术进一步筛选出高效表达克隆，建立稳定高效表达CHO细胞株。
Objective:To establish a stable CHO cell line expressing GLUT4-EGFP, and to lay a foundation for investigating the translocation mechanisms of GLUT4 in CHO cells.
目的：建立稳定表达EGFP标记的葡萄糖转运蛋白4的CHO细胞系，为研究GLUT4在CHO细胞中的转运调节机制奠定基础。
Objective To construct eukaryotic expressing vector of human melanin-concentrating hormone receptor 1(MCHR1), then to transfect CHO cells with the vector for establishment of stable CHO cell line.
目的构建人黑色素浓集激素1型受体（MCHR1）真核表达载体，转染CHO细胞，建立稳定转染的CHO细胞系。
Objective: To optimize the condition of gene transient transfection in CHO-K1 cell.
目的：以CHO - K1细胞为宿主基因瞬时转染条件的优化。
The ultrastructure of prematurely condensed chromosomes (PCC) in BK cells and the CHO metaphase chromosomes (the PCC inducer) were studied with cell fusion technique and SEM.
应用细胞融合技术和扫描电镜研究了BK(牛肾)细胞早熟凝集染色体(PCC)和诱导PCC的CHO中期染色体的超微结构。
The ultrastructure of prematurely condensed chromosomes (PCC) in BK cells and the CHO metaphase chromosomes (the PCC inducer) were studied with cell fusion technique and SEM.
应用细胞融合技术和扫描电镜研究了BK(牛肾)细胞早熟凝集染色体(PCC)和诱导PCC的CHO中期染色体的超微结构。
应用推荐