To clarify the regulatory mechanism, site-directed mutagenesis and reporter gene assay in the CHO cell line were used.
为了阐明调控机制，我们采用了点突变实验和在CHO细胞系中的报告基因分析。
In comparison with commercial SFM, it can maintain growth of CHO cell for much longer peroid and the expressing level of HBsAg is higher too.
该培养基与商品化的无血清培养基比较，能够使细胞生长维持较长的时间，表达产物分泌量也相对较高。
Objective:To establish a stable CHO cell line expressing GLUT4-EGFP, and to lay a foundation for investigating the translocation mechanisms of GLUT4 in CHO cells.
目的：建立稳定表达EGFP标记的葡萄糖转运蛋白4的CHO细胞系，为研究GLUT4在CHO细胞中的转运调节机制奠定基础。
Objective To construct eukaryotic expressing vector of human melanin-concentrating hormone receptor 1(MCHR1), then to transfect CHO cells with the vector for establishment of stable CHO cell line.
目的构建人黑色素浓集激素1型受体（MCHR1）真核表达载体，转染CHO细胞，建立稳定转染的CHO细胞系。
Objective To construct eukaryotic expressing vector of human melanin-concentrating hormone receptor 1(MCHR1), then to transfect CHO cells with the vector for establishment of stable CHO cell line.
目的构建人黑色素浓集激素1型受体（MCHR1）真核表达载体，转染CHO细胞，建立稳定转染的CHO细胞系。
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